US 2022/0024969 A1Published application

Method for extracting astragaloside iv from fresh radix astragali

AssigneesShanxi University, Fukanglihua (Shanxi) Biological Technology Co., Ltd.

InventorsJigang PAN, Yuejun HUANG, Jun ZHANG

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Abstract

The disclosure discloses a method for extracting Astragaloside IV from fresh Radix Astragali. The method comprises the following steps: cleaning and cutting fresh Radix Astragali roots into sections, and extracting with an ethanol solution for multiple times under an ultrasonic condition; merging the extracting solutions, concentrating to remove ethanol, hydrolyzing the concentrate with sodium hydroxide-calcium hydroxide mixed alkali, filtering the precipitate; then flocculating and precipitating by using a flocculating agent chitosan, and filtering; removing impurities through a flocculation-precipitation mode and filtering with a filter screen; separating through a macroporous adsorption resin, and finally purifying through recrystallization to obtain a high-purity Astragaloside IV. The method has the advantages of easily available raw materials, low cost, high Astragaloside IV extraction efficiency, high Astragaloside IV purity and simple process, the raw materials involved in the extraction process are non-toxic and harmless, and it is suitable for large-scale industrial production.

Claims9

Dependent claims

A method for extracting Astragaloside IV from fresh Radix Astragali, comprising the following steps:

(a) Cleaning the fresh Radix Astragali roots, and cutting the roots into sections;
(b) Under ultrasonic conditions, extracting several times with an ethanol solution to obtain an extract, concentrating under reduced pressure to remove ethanol to obtain a concentrate, adding water to the concentrate to 4-8 times the weight of the fresh Radix Astragali, and filtering to obtain a concentrated solution;
(c) Adjusting the pH of the concentrated solution to maintain at 11-13 with sodium hydroxide-calcium hydroxide mixed alkali under a condition of 40-60° C., keeping temperature and hydrolyzing for 2-4 hours, filtering with a filter screen, and collecting a filtrate;
(d) Adjusting a pH of the filtrate with an acid to 5-7, then adding a chitosan solution for flocculation, filtering with a filter screen, and collecting a filtrate;
(e) Separating the obtained filtrate through a macroporous adsorption resin column to obtain a crude Astragaloside IV;
and
(f) Recrystallizing the crude Astragaloside IV with methanol or ethanol to obtain a high-purity Astragaloside IV.

4 more elements

Dependencies are inferred from the claim text.