Centrifugal separator

A centrifugal separator for separation of a cell culture mixture, comprising a stationary frame, a rotatable assembly and a drive unit for rotating the rotatable assembly relative the frame around an axis of rotation,

• wherein the rotatable assembly comprises:
• a rotor casing enclosing a separation space in which a stack of separation discs is arranged to rotate around a vertical axis of rotation, said rotor casing further comprising a mechanically hermetically sealed inlet for supply of said cell culture mixture to said separation space;
• a first mechanically hermetically sealed liquid outlet for discharge of a separated liquid phase;
• and
• a second mechanically hermetically sealed liquid outlet for discharge of a separated cell phase, said cell phase having a density that is higher than said liquid phase;
• phase,
• wherein said hermetically sealed inlet is arranged at a first axial end of said rotor casing and arranged so that the cell culture mixture to be separated enters said rotor casing at the rotational axis,
• wherein said second mechanically hermetically sealed outlet is arranged at a second axial end of said rotor casing opposite said first end and arranged so that said separated cell phase is discharged at the rotational axis, and
• wherein the rotatable assembly comprises an exchangeable separation insert and a rotatable member, said insert comprising said rotor casing and being supported by said rotatable member.