Point of care (poc) device for facilitating nucleic acid based testing and method thereof

A point of care (POC) based device for use in the detection of pathogenic infection via nucleic acid based testing comprising:

• a thermal control cum reaction unit including a microcontroller based isothermal heating unit;
• at least one movable reaction microchamber hosting desired samples and reagents including labelled primer and probe sets adapted for seamless integration to a first position in said thermal control cum reaction unit having said isothermal heating unit
• for executing piecewise-isothermal reaction steps selectively for carrying out in said at least one movable anyone or more of (a) RNA containing test sample conditioning (b) reverse transcription to c-DNA including its amplification, and (c) complementary DNA-probe hybridization in said reaction microchamber under selectively controlled isothermal heating piecewise and selectively following as required said microcontroller controlled isothermal heating in stages including selectively (a) raising temperature to a desired level (b) holding temperature to a desired temperature for a specified time and (c) lowering temperature to a desired level for desired seamlessly integrated and selective (a) RNA containing test sample conditioning (b) reverse transcription to c-DNA including its amplification, and (c) complementary DNA-probe hybridization adapted to generate desired DNA-probe hybridized reaction product in said at least one movable reaction microchamber;
• said thermal control cum reaction unit having at a second position therein an on-line dispenser mechanism for dispensing generated reaction products from said movable reaction microchamber,
• a seamlessly integrated and guided movable POC detector unit including a colorimetric detection unit adapted for complementary analyte binding/hybridization reaction product disposed in said second position within said thermal control cum reaction unit for cooperative disposition thereunder said movable reaction microchamber containing said generated reaction products for on-line sequentially dispensing of the amplified and labelled cDNA product and/or test reagents as said reaction product onto said POC detector unit for desired piecewise and contamination free detection of pathogenic infection from respective movable reaction microchamber.