Microfluidic siphoning array for nucleic acid quantification
AssigneeCombinati Incorporated
InventorsJu-Sung Hung, Andrew Zayac, Megan Dueck
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Abstract
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
Claims20
A device for processing a biological sample, comprising:
- a plurality of chambers configured to retain said biological sample during processing;
- and
- at least one gas permeable film or membrane covering said plurality of chambers, wherein said gas permeable film or membrane is configured to permit gas flow from a chamber of said plurality of chambers to a location external to said chamber.
A device for processing a biological sample, comprising a plurality of chambers configured to retain said biological sample during processing, wherein a chamber of said plurality of chambers comprises a barrier that is configured to permit gas flow from a chamber of said plurality of chambers to a location external to said chamber.
Dependencies are inferred from the claim text.