Adeno-associated virus (aav) clades, sequences, vectors containing same, and uses therefor
AssigneeThe Trustees of the University of Pennsylvania
InventorsJames M. Wilson, Guangping Gao, Mauricio R. Alvira, Luc H. Vandenberghe
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Abstract
Sequences of novel adeno-associated virus capsids and vectors and host cells containing these sequences are provided. Also described are methods of using such host cells and vectors in production of rAAV particles. AAV-mediated delivery of therapeutic and immunogenic genes using the vectors of the invention is also provided.
Claims12
A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp1 capsid protein having a sequence comprising amino acids 1 to 738 of SEQ ID NO:
- 88 (AAVhu37), wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence, and wherein the recombinant nucleic acid molecule is a plasmid.
A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp2 capsid protein having a sequence comprising amino acids 138 to 738 of SEQ ID NO:
- 88 (AAVhu37), wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence, and wherein the recombinant nucleic acid molecule is a plasmid.
A cultured host cell containing a recombinant nucleic acid molecule encoding an AAV vp3 capsid protein having a sequence comprising amino acids 204 to 738 of SEQ ID NO:
- 88 (AAVhu37), wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence, and wherein the recombinant nucleic acid molecule is a plasmid.
A cultured host cell containing a recombinant nucleic acid molecule comprising (a) nucleotides 1 to 2214 of SEQ ID NO:
- 10, (b) nucleotides 412 to 2214 of SEQ ID NO:
- 10, or (c) nucleotides 610 to 2214 of SEQ ID NO:
- 10, wherein the recombinant nucleic acid molecule further comprises a heterologous non-AAV sequence, and wherein the recombinant nucleic acid molecule is a plasmid.
Dependencies are inferred from the claim text.