Pars imaging methods
AssigneeIllumisonics Inc.
InventorsParsin HAJI REZA, Zohreh HOSSEINAEE, Kevan BELL, Saad ABBASI, Ben ECCELSTONE
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Abstract
A method for visualizing details in a sample including directing an excitation beam to an excitation location below a surface of the sample, to generate signals in the sample; directing an interrogation beam toward the excitation location of the sample; directing a signal enhancement beam to the sample, to raise a temperature of a portion of the sample by 5 Kelvin or less, compared to a temperature of the portion of the sample in absence of the signal enhancement beam; detecting a portion of the interrogation beam returning from the sample that is indicative of the generated signals.
Claims23
A method for visualizing details in a sample, the method comprising:
- directing an excitation beam to an excitation location below a surface of the sample, to generate signals in the sample;
- directing an interrogation beam toward the excitation location of the sample;
- directing a signal enhancement beam to the sample, to raise a temperature of a portion of the sample, compared to a temperature of the portion of the sample in absence of the signal enhancement beam, wherein the portion of the sample is within a focal point of the interrogation beam;
- and
- detecting a portion of the interrogation beam returning from the sample that is indicative of the generated signals.
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9-12. (canceled)
A method for visualizing details in a sample, the method comprising:
- directing an excitation beam to an excitation location below a surface of the sample, to generate signals in the sample;
- directing an interrogation beam toward the excitation location of the sample;
- extracting absorption signals from a returning portion or portions of the interrogation beam;
- determining scattering intensity from the returning portion or portions of the interrogation beam;
- and
- generating a combined image from the extracted absorption signals and the determined scattering intensity.
14-27. (canceled)
A method for visualizing details in a sample, the method comprising:
- generating pressure, temperature, and fluorescence signals in the sample at an excitation location using an excitation beam, the excitation beam being focused on the sample;
- interrogating the sample with an interrogation beam directed toward the excitation location of the sample, the interrogation beam being focused on the sample;
- detecting at least a portion of the interrogation beam returning from the sample, the returned portion of the interrogation beam being indicative of the generated pressure and temperature signals;
- and
- detecting fluorescence signals from the excitation location of the sample, simultaneously with detecting the generated pressure and temperature signals.
Dependencies are inferred from the claim text.