Compositions and methods for carrying out assay measurements
AssigneeMeso Scale Technologies, LLC.
InventorsAlexander K. TUCKER-SCHWARTZ, George SIGAL
Life of this patent
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Abstract
The present disclosure provides compositions, reagents, kits, systems, system components, and methods for performing assays. More particularly, the disclosure relates to an assay composition for detecting luminescence, which comprises an alkyl diethanolamine, for example, N-butyldiethanolamine (BDEA). In embodiments, the assay composition comprises 2-dibutylaminoethanol (BDAE).
Claims114
A composition comprising:
- (a) (i) N-butyldiethanolamine (BDEA) or 2-dibutylaminoethanol (DBAE) or both, (ii) a pH buffering component, and (iii) a salt;
- or
- (b) (i) N-butyldiethanolamine (BDEA) or 2-dibutylaminoethanol (DBAE) or both, (i) a pH buffering component, (iii) a salt;
- and (iv) a surfactant;
- or
- (c) (i) N-butyldiethanolamine (BDEA) or 2-dibutylaminoethanol (DBAE) or both, (ii) a pH buffering component, (iii) a salt, and (iv) a liquid diluent;
- or
- (d) (i) N-butyldiethanolamine (BDEA) or 2-dibutylaminoethanol (DBAE) or both, a pH buffering component, (iii) a salt;
- (iv) a surfactant;
- and (v) a liquid diluent,
- wherein the composition has an ionic strength of greater than about 0.3 M.
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A kit comprising, in one or more containers:
- (a) N-butyldiethanolamine (BDEA) or 2-dibutylaminoethanol (DBAE), or both;
- (b) a pH buffering component;
- and
- (c) a salt,
- wherein a composition formed from combining components of the kit has an ionic strength of greater than about 0.3 M.
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A method for producing a composition comprising combining
- (i) N-butyldiethanolamine (BDEA) or 2-dibutylaminoethanol (DBAE), or both;
- (ii) a pH buffering component;
- and
- (iii) a salt,
- wherein the composition has an ionic strength of greater than about 0.3 M.
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A composition comprising:
- (i) about 100 mM to about 150 mM N-butyldiethanolamine (BDEA), 2-dibutylaminoethanol (DBAE), or both;
- (ii) about 250 mM to about 1400 mM of a salt;
- (iii) a pH buffering component;
- and
- (iv) a surfactant selected from KOLLIPHOR P-407, PLURONIC P-123, PLURONIC L-121, PLURONIC 31R1, TETRONIC 701, 2,4,7,9-tetramethyl-d-decyne-4,7-diol ethoxylate, PEG(18) tridecyl ether, BRIJ L4, BRIJ 58, TWEEN 20, or a combination thereof.
Dependencies are inferred from the claim text.