Method for the Detection of Surface-Mounted Biological Materials and Pathogens
AssigneeNanoco Technologies Ltd.
InventorImad Naasani
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Abstract
Methods and compositions for the detection of surface-mounted pathogens are described herein. Compositions include preparations comprising quantum dot-ligand conjugates, wherein the ligands target a specific pathogen to form a quantum dot-pathogen complex. Methods include the use of the preparations comprising the quantum dot-ligand conjugates. The preparations may be applied to a surface for the detection of a surface-mounted pathogen thereon via fluorescence, which may be detected by the naked eye or a simple fluorescence camera.
Claims20
A method for detecting a pathogen in a tissue sample or bodily fluid, the method comprising,
- providing a composition comprising a population of quantum dot (QD)-ligand conjugates, the population of QD-ligand conjugates comprising:
- a first population of QD-ligand conjugates configured to absorb light from a UV-of blue-emitting light source and emit light at a first wavelength;
- and
- a second population of QD-ligand conjugates configured to absorb light from a UV- or blue-emitting light source and emit light at a first wavelength;
- contacting the composition comprising the population of quantum dot (QD)-ligand conjugates with a tissue sample or a bodily fluid containing a pathogen to bond one or more of the QD-ligand conjugates to the pathogen and form QD-pathogen complexes, and
- subjecting the QD-pathogen complexes to irradiation with a light source.
A method for detecting a pathogen in a tissue sample or bodily fluid, the method comprising,
- providing a composition comprising a quantum dot (QD)-ligand conjugate, the QD-ligand conjugate comprising:
- a first type of ligand covalently bound to the quantum dot;
- and
- a second type of ligand reversibly bound to the quantum dot;
- contacting the composition comprising the quantum dot (QD)-ligand conjugate with a tissue sample or a bodily fluid containing a pathogen to bond the QD-ligand conjugate to the pathogen and form a QD-pathogen complex, and
- subjecting the QD-pathogen complex to irradiation with a light source.
Dependencies are inferred from the claim text.