Method for eliminating interference of theophylline in immunoassay and immunoassay kit

An immunoassay method, comprising:

• providing a blood sample;
• providing an immunoassay kit, wherein the immunoassay kit comprises a substrate, a labeling substance, and a solid phase coated with an immunoassay reactant, wherein at least one of the substrate, the labeling substance and the solid phase comprises a theophylline interference-resistant agent, and the theophylline interference-resistant agent comprises at least one component selected from the group consisting of 6-mercaptopurine, 6-thioguanine, 8-azaguanine, hypoxanthine, adenine, purine, 2,6,8-trioxypurine, adenine nucleotide and adenosine;
• performing an immunoassay of the blood sample by using the immunoassay kit, wherein the theophylline interference-resistant agent has a final concentration of 10 mg/mL to 100 mg/mL and is used to eliminate interference of theophylline in the immunoassay of the blood sample.

A method for eliminating interference of theophylline in immunoassay of a sample, comprising:

• performing an immunoassay reaction in the presence of a theophylline interference-resistant agent, wherein the theophylline interference-resistant agent has a final concentration of 0.1 mg/mL to 100 mg/mL, and comprises at least one component selected from the group consisting of 6-mercaptopurine, 6-thioguanine, 8-azaguanine, hypoxanthine, adenine, purine, 2,6,8-trioxypurine, adenine nucleotide and adenosine.