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US 2022/0018779 A1Published application
Method, system and application for 3d molecular diffusion tensor measurement and structural imaging
AssigneeClemson University
InventorsHAI YAO, TONG YE, PENG CHEN, XUN CHEN
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Abstract
Herein are described data acquisition systems and methods applying such systems to determine three-dimensional (3D) diffusion tensors, and simultaneously, to perform 3D structure imaging. Example data acquisition systems can include computing systems in communication with modified light sheet microscopes that are configured for high-speed volumetric imaging to record 3D diffusion processes and high-resolution 3D structural imaging.
Claims22
A data acquisition system comprising:
- a first laser configured to transmit an illumination beam along an illumination path and direct the illumination beam to irradiate a 3D volume of a sample area;
- a bleaching unit configured to direct a bleaching beam along a bleaching path and contact a portion of the 3D volume of the sample area with the bleaching beam;
- and
- a sensor configured to detect a first series of emissions emitted within the 3D volume corresponding to a 3D illumination response signal and a second series of emissions emitted within the 3D volume at a later time and corresponding to a 3D bleached signal;
- wherein
- analysis of the first series of emissions and the second series of emissions provides a full 3D diffusion tensor of a molecular species retained in the sample area.
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A non-invasive method for determining a 3D diffusion tensor for a molecular species in a material, the method comprising:
- irradiating a 3D volume comprising the molecular species in the material with an illumination beam;
- bleaching a portion of the 3D volume with a bleaching beam;
- obtaining emission data from the 3D volume, wherein the emission data comprises a first series of emissions generated by the material upon exposure of the volume to the illumination beam and comprises a second series of emissions generated by the material after exposure of the portion of the volume to a bleaching beam;
- analyzing the emission data, the analysis comprising a 3D spatial Fourier analysis of the emission data;
- and
- determining, based at least in part on the 3D spatial Fourier analysis, a full 3D diffusion tensor for the molecule in the material.
3 more elements
Dependencies are inferred from the claim text.