Processing and imaging tissue samples
AssigneeAkoya Biosciences, Inc.
InventorMichael McLane
Life of this patent
Estimating the term…
Abstract
Methods include applying a first stain composition comprising a fluorescent counterstain to a biological sample, measuring information corresponding to one or more stains of the first stain composition, removing the fluorescent counterstain from the biological sample, applying a second stain composition to the biological sample, measuring information corresponding to one or more stains of the second stain composition in the biological sample, where the one or more stains include a fluorescent label, and generating a first image of the biological sample, where the first image corresponds to a pattern of simulated hematoxylin staining in the biological sample.
Claims30
A method, comprising:
- applying a first stain composition to a biological sample, and measuring information corresponding to one or more stains of the first stain composition in the biological sample, wherein the one or more stains comprise a fluorescent counterstain;
- removing the fluorescent counterstain from the biological sample;
- applying a second stain composition to the biological sample, and measuring information corresponding to one or more stains of the second stain composition in the biological sample, wherein the one or more stains comprise a fluorescent label;
- generating a first image of the biological sample, wherein the first image corresponds to a pattern of simulated hematoxylin staining in the biological sample;
- and
- generating a second image of the biological sample, wherein the second image corresponds to localization of the fluorescent label in the biological sample.
A method, comprising:
- applying a first stain composition to a biological sample that comprises at least one of curcumin and carmine lake;
- applying a second stain composition to the biological sample that comprises at least one of eosin and indigo carmine;
- measuring image information corresponding to the first and second stain compositions in the biological sample;
- exposing the biological sample to at least one antigen retrieval agent to remove the at least one of curcumin and carmine lake and the at least one of eosin and indigo carmine from the biological sample;
- applying a third stain composition to the biological sample, wherein the third stain composition comprises a fluorescent label;
- measuring image information corresponding to the third stain composition in the biological sample;
- and
- generating a first image based on the image information corresponding to the first and second stain compositions in the biological sample that represents a hematoxylin and eosin staining distribution in the biological sample.
Dependencies are inferred from the claim text.