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US 2022/0017954 A1Published application
Methods for Preparing CDNA Samples for RNA Sequencing, and CDNA Samples and Uses Thereof
AssigneeQiagen Sciences, LLC
InventorsYexun WANG, Marcus LEWIS
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Abstract
The invention relates to methods for preparing cDNA samples for RNA sequencing using random priming oligonucleotides comprising a cell barcode (cID), a unique molecular index (UMI), and a random sequence region, and performing a reverse transcription reaction (RT). The invention also relates to cDNA samples prepared by the methods and uses thereof.
Claims26
A method of generating a cDNA sample, comprising
- adding to an RNA sample, a plurality of random priming oligonucleotides, each random priming oligonucleotide comprising a cell barcode (cID), a unique molecular index (UMI), and a random sequence region of 5 to 8 nucleotides, and
- performing a reverse transcription reaction (RT).
A random priming oligonucleotide comprising a random sequence region of 5 to 8 nucleotides, a unique molecular index (UMI), and a cell barcode (cID).
Dependencies are inferred from the claim text.