Methods, compositions, and systems for mapping locations of single molecules in multi-dimensional space

A method for mapping locations of targets in multi-dimensional space, the method comprising:

• establishing a distribution of functionalized particles in space and in proximity to a sample comprising a distribution of a set of targets;
• promoting selective capture of the set of targets by a first subset of molecules coupled to the distribution of functionalized particles;
• promoting interactions between active regions of a second subset of molecules coupled to the distribution of functionalized particles, the active regions of a functionalized particle configured to preferentially interact with the active regions of neighboring functionalized particles of the distribution of functionalized particles;
• and
• for each of the distribution of functionalized particles:
• identifying connectivity of a subset of neighboring particles of a respective functionalized particle based upon interactions between active regions of the respective functionalized particle and active regions of the subset of neighboring particles;
• and
• identifying a subset of targets captured by the respective functionalized particle.