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AssigneeCodiak BioSciences, Inc.
InventorsKevin P. DOOLEY, Rane A. HARRISON, Ke XU, Damian J. HOUDE, Sonya HAUPT, John D. KULMAN, Douglas E. WILLIAMS, Russell E. MCCONNELL, Madeleine YOUNISS
Estimating the term…
The present disclosure relates to therapeutic exosomes enriched in proteins that are present in the luminal surface of exosomes. The present disclosure provides methods of manufacturing exosomes enriched in proteins that are present in the luminal surface of exosomes, method of associating a therapeutic peptide or protein to the luminal surface of exosomes, and method of use, e.g., methods of therapeutic or diagnostic use. The methods of manufacture involve generating of luminal-surface-engineered exosomes that include one or more of the EV, e.g., exosome proteins at concentrations higher that those observed in wild type exosomes, a modification or a fragment of the EV, e.g., exosome protein, or a fusion protein of the EV, e.g., exosome protein, and a payload, e.g., biologically active molecule such as a therapeutic protein.
An isolated extracellular vesicle (EV) comprising a biologically active molecule linked to a scaffold protein, wherein the scaffold protein comprises an N-terminus domain (ND) and an effector domain (ED), wherein the ND is associated with the luminal surface of the EV and the ED is associated with the luminal surface of the EV by an ionic interaction, wherein the ED comprises at least two contiguous lysines (Lys) in sequence.
An isolated extracellular vesicle (EV) comprising a biologically active molecule linked to a scaffold protein, wherein the scaffold protein comprises an N-terminus domain (ND) and an effector domain (ED), wherein the ND comprises the amino acid sequence as set forth in G:X2:X3:X4:X5:X6, wherein G is a glycine, represented by Gly;
An isolated extracellular vesicle (EV) comprising a biologically active molecule linked to a scaffold protein, wherein the scaffold protein comprises ND-ED, wherein:
Dependencies are inferred from the claim text.