Automated nucleic acid extraction method and device

An automated nucleic acid extraction method applied to an automated nucleic acid extraction device, wherein the automated nucleic acid extraction device comprises a base body, a cassette, a driving unit, a moving frame and a syringe;

• the base body comprises a sample accommodating area, a column accommodating area, a cassette accommodating area and a collection tube, the sample accommodating area, the column accommodating area, the cassette accommodating area and the collection tube are arranged in a linear direction;
• the cassette is arranged in the cassette accommodating area, the cassette includes two parallel walls and at least two vertical walls, the parallel walls and the vertical walls jointly form a lysis buffer well, at least one wash buffer well and an elution buffer well, each of the vertical walls forming the lysis buffer well, the wash buffer wells and the elution buffer well includes a load-bearing abutment, the lysis buffer well, the wash buffer well and the elution buffer well are arranged in the linear direction, and the load-bearing abutment is provided with an arcuate wall or a polygonal wall;
• the driving unit is arranged on the base body;
• the moving frame is arranged on the base body vertically and driven by the driving unit to reciprocate in the linear direction;
• and the syringe is arranged on the moving frame and is moved along with the moving frame, the automated nucleic acid extraction method comprising following steps of:
• using the syringe in cooperate with a column tip to suck a mixed reactant in the binding buffer accommodating space, so that nucleic acids contained in the reactant are bound to a membrane inside the column tip, and to eject a reaction residue to the cassette, so that nucleic acids inside the reaction residue are bound to the membrane;
• using the syringe in cooperate with the column tip to suck the wash buffer in the at least one wash buffer well through the membrane, and then using the syringe in cooperate with the column tip to eject the wash buffer through the membrane;
• and
• using the syringe in cooperate with the column tip to suck the elution buffer in the elution buffer well through the membrane, and then using the syringe in cooperate with the column tip to eject through the membrane the elution buffer containing the nucleic acids to the collection tube.