Compositions and methods for modifying a target nucleic acid
AssigneeThe Regents of the University of California
InventorsAlexander Marson, Theodore Lee Roth, Daniel Goodman, David-Huy Nhu Nguyen, Francis C. Szoka
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Abstract
The disclosure provides compositions and methods for modifying a target nucleic acid. In some embodiments, a composition can include a targetable nuclease, a DNA-binding protein, and a donor template comprising a homology directed repair (HDR) template and one or more DNA-binding protein target sequences. In some embodiments, a composition can include a Cas protein, one or more single guide RNAs (sgRNAs), and an anionic polymer.
Claims120
A composition for modifying a target nucleic acid, comprising:
- (a) a targetable nuclease;
- (b) a DNA-binding protein;
- (c) a donor template comprising a homology directed repair (HDR) template and one or more DNA-binding protein target sequences.
A composition for modifying a target nucleic acid, comprising:
- (a) a Cas protein;
- and
- (b) an anionic polymer.
A ribonucleoprotein complex for modifying a target nucleic acid, comprising a Cas protein, one or more sgRNAs, and an anionic polymer.
A method for modifying a target nucleic acid in a cell, comprising introducing into the cell:
- (a) a Cas protein;
- (b) an anionic polymer;
- and
- (c) a viral vector comprising an sgRNA.
A method of forming a ribonucleoprotein (RNP) complex, comprising incubating a Cas protein, an sgRNA, and an anionic polymer in a mixture.
A composition comprising a donor template comprising a homology directed repair (HDR) template and one or more DNA-binding protein target sequences.
Dependencies are inferred from the claim text.