Human Pluripotent Stem Cell-Based Models for Predictive Developmental Neural Toxicity
AssigneeWisconsin Alumni Research Foundation
InventorsJames A. Thomson, William L. Murphy, Charles D. Page, Michael P. Schwartz, Zhonggang Hou
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Abstract
The present invention relates to three-dimensional (3D) tissue constructs and methods of using such 3D tissue constructs to screen for neurotoxic agents. In particular, provided herein are methods of producing and using complex, highly uniform human tissue models comprising physiologically relevant human cells, where the tissue models have the degree of sample uniformity and reproducibility required for use in quantitative high-throughput screening applications.
Claims16
1-26. (canceled)
A method of producing a vascularized neural tissue construct, comprising
- (a) seeding a three-dimensional porous material with human neural progenitor cells;
- (b) culturing the seeded material for a length of time sufficient to detect differentiation of at least a portion of the neural progenitor cells;
- (c) dispersing, on or within the cultured seeded material, human endothelial cells;
- and
- (d) culturing the seeded material comprising the dispersed human endothelial cells under culture conditions that promote cell differentiation, whereby a vascularized neural tissue construct comprising human neurons and glial cells is produced,
- wherein the porous material is proteolytically degradable.
Dependencies are inferred from the claim text.