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AssigneeKanto Kagaku Kabushiki Kaisha
InventorsTakehiro WATANABE, Tomoya SAWAGUCHI, Megumi YAMAZAKI
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The present invention addresses the problem of providing: a culture medium that is for adherent culturing of pluripotent stem cells exhibiting high growth ability and that does not contain serum or albumin; and a highly versatile method for adherent culturing of pluripotent stem cells using the culture medium. According to the present invention, by adding, to a culture medium, a hydrophilic polymer that is conventionally known to inhibit adhesion of cells to the surface of a cell culture base material, so that the adhesion of pluripotent stem cell to the surface of the cell culture base material is enhanced, high growth ability is imparted in adherent culturing of pluripotent stem cells without adding serum or albumin thereto. In addition, by adding an antioxidant substance along with such a polymer to a culture medium, very high growth ability is imparted in adherent culturing of pluripotent stem cells.
A medium for adhesive culture of a pluripotent stem cell, comprising a hydrophilic polymer but substantially no albumin.
A medium for adhesive culture of a pluripotent stem cell, comprising a hydrophilic polymer, an antioxidant, and a differentiation suppressing agent consisting of a GSK3β inhibitor and a DYRK inhibitor.
A method for enhancing the adherence of a pluripotent stem cell to a culture substrate, comprising adding a hydrophilic polymer into a medium.
Dependencies are inferred from the claim text.