Endodermal cell population, and method for producing cell population of any of three germ layers from pluripotent cell
AssigneesKaneka Corporation, National Center for Global Health and Medicine
InventorsMasato IBUKI, Hitoshi OKOCHI, Shigeharu YABE
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Abstract
An object of the present invention is to provide an endodermal cell population for obtaining optimal somatic cells as cell therapy preparations. The endodermal cell population of the present invention has a reduced content proportion of undifferentiated cells in the cell population and contains endodermal cells differentiable into optimal somatic cells as cell therapy preparations. Further, a somatic cell derived from the endodermal cell population of the present invention has excellent therapeutic effects as a cell therapy preparation.
Claims21
An endodermal cell population, wherein a relative expression level of a Nanog gene to an expression level of an OAZ1 gene is 0.8 or less, a relative expression level of a HNF1B gene to an expression level of the OAZ1 gene is 1.0 or more, and a proportion of SOX17-positive cells is 80% or more.
A method for producing an endodermal cell from a pluripotent stem cell, the method comprising the following (a) to (b):
- (a) a step of suspension culture of a pluripotent stem cell using a medium containing 2-mercaptoethanol to prepare a cell population, and
- (b) a step of culturing the cell population using a medium containing a TGFβ superfamily signaling activator, and subsequently culturing using a medium to which FGF2 and BMP4 are not added.
A method for producing a cell population of any of three germ layers from a pluripotent stem cell, the method comprising the following (a) to (b):
- (a) a step of suspension culture of a pluripotent stem cell using a medium containing 2-mercaptoethanol to prepare a cell population, and
- (b) a step of culturing the cell population under conditions that allow the cell population to be induced to differentiate into any of an endodermal cell, a mesodermal cell, or an ectodermal cell.
Dependencies are inferred from the claim text.