Production methods for megakaryocytes and platelets
AssigneeKyoto University
InventorKoji ETO
Life of this patent
Estimating the term…
Abstract
An object of the present invention is to provide a method of efficiently producing a maturated megakaryocytic cell line from hematopoietic progenitor cells. The present invention provides a method for producing megakaryocytes from hematopoietic progenitor cells, comprising (i) forcibly expressing an apoptosis suppression gene and an oncogene in hematopoietic progenitor cells and culturing the cells, and (ii) arresting forced expression of the apoptosis suppression gene and the oncogene and culturing the hematopoietic progenitor cells.
Claims12
1-36. (canceled)
A method for producing megakaryocytes from hematopoietic progenitor cells, comprising the following (i) and (ii) steps:
- (i) forcibly expressing BCL-XL gene and c-MYC gene in hematopoietic progenitor cells and culturing the cells, and
- (ii) arresting the forced expression of the BCL-XL gene and the c-MYC gene in the cells obtained in the step (i) and culturing the cells.
A method for producing megakaryocytes from hematopoietic progenitor cells, comprising the following (i) and (ii) steps:
- (i) forcibly expressing c-MYC gene in hematopoietic progenitor cells and culturing the cells in a medium containing Z-DEVD-FMK;
- and
- (ii) arresting the forced expression of the c-MYC gene in the cells obtained in the step (i) and culturing the cells.
Dependencies are inferred from the claim text.