New lactic acid bacteria
AssigneeDupont Nutrition Biosciences Aps
InventorsArmelle COCHU-BLACHÈRE, Christophe FREMAUX, Thomas DESFOUGÈRES, Anaïs JEDRZEJOWSKI
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Abstract
The invention relates to a lactose-positive, galactose-negative, Streptococcus thermophilus strain carrying a mutation in 2 or 3 genes selected from the group consisting of 1) a gene encoding a protein of the mannose-glucose-specific PTS and a glcK gene, 2) a gene encoding a protein of the mannose-glucose-specific PTS and a ccpA gene, and 3) a gene encoding a protein of the mannose-glucose-specific PTS, a glcK gene and a ccpA gene, wherein said strain, when used to ferment milk, provides a low lactose fermented milk and/or a fermented milk not undergoing acidification when stored at fermentation temperature.
Claims22
A lactose-positive, galactose-negative, Streptococcus thermophilus strain carrying a mutation in genes selected from the group consisting of 1) at least one gene encoding a protein of the mannose-glucose-specific PTS and a glcK gene, 2) at least one gene encoding a protein of the mannose-glucose-specific PTS and a ccpA gene, and 3) at least one gene encoding a protein of the mannose-glucose-specific PTS, a glcK gene and a ccpA gene;
- wherein, when said strain is used to ferment milk as assayed by test B, the ratio of the amount of galactose released (mM) over the amount of remaining lactose (mM) in said fermented milk is more than 1.2.
(canceled)
A Streptococcus thermophilus ccpA polynucleotide, selected from the group consisting of:
- a) a ccpA polynucleotide, which when inserted in lieu of the ccpA gene of the DGCC7710 strain, leads to a DGCC7710 derivative exhibiting a ratio of beta-galactosidase activity as assayed by test D over the glucokinase activity as assayed by test E of at least 4.10−6;
- b) a ccpA polynucleotide, which when inserted in lieu of the ccpA gene of a DGCC7710 strain into which the manL gene has previously been replaced by a manL gene as defined in SEQ ID NO:111 (i.e., of the DGCC7710-IIABMan305 strain) leads to a DGCC7710-IIABMan305 derivative, which, when used to ferment milk as assayed by test B, exhibits a ratio of the amount of galactose released (mM) over the amount of remaining lactose (mM) in said fermented milk which is more than 1.2, more than 1.5, more than 2, more than 2.5 or more than 3;
- c) a ccpA polynucleotide, which when inserted in lieu of the ccpA gene of a DGCC7710 strain into which the manM gene has previously been replaced by a manM gene as defined in SEQ ID NO:157 (i.e., of the DGCC7710-IICMan208 strain) leads to a DGCC7710-IICMan208 derivative, which, when used to ferment milk as assayed by test B, exhibits a ratio of the amount of galactose released (mM) over the amount of remaining lactose (mM) in said fermented milk which is more than 1.2, more than 1.5, more than 2, more than 2.5 or more than 3;
- and
- d) a ccpA polynucleotide, which when inserted in lieu of the ccpA gene of a DGCC7710 strain into which the manN gene has previously been replaced by a manN gene as defined in SEQ ID NO:206 (i.e., of the DGCC7710-IIDMan28 strain) leads to DGCC7710-IIDMan28 derivative, which, when used to ferment milk as assayed by test B, exhibits a ratio of the amount of galactose released (mM) over the amount of remaining lactose (mM) in said fermented milk which is more than 1.2, more than 1.5, more than 2, more than 2.5 or more than 3.
-30. (canceled)
Dependencies are inferred from the claim text.