Tumor-specific anti-egfr antibody and application thereof

A multi-functional immunoconjugate, comprising:

• an antibody;
• and
• a functional molecule linked thereto;
• and the functional molecule is selected from a group consisting of a molecule that targets a tumor surface marker, a tumor-suppressing molecule, a molecule that targets a surface marker of an immune cell, a detectable label;
• wherein the antibody specifically recognizing EGFRvIII expressed or EGFR overexpressed by tumor cells, wherein the antibody comprises a light chain variable region and a heavy chain variable region, and
• CDR1 of the light chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 41, SEQ ID NO:
• 47, SEQ ID NO:
• 55;
• CDR2 of the light chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 42, SEQ ID NO:
• 53;
• CDR3 of the light chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 43, SEQ ID NO:
• 48, SEQ ID NO:
• 54, SEQ ID NO:
• 56, SEQ ID NO:
• 57;
• CDR1 of the heavy chain variable region has the amino acid sequence of SEQ ID NO:
• 44;
• CDR2 of the heavy chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 45, SEQ ID NO:
• 49, SEQ ID NO:
• 51, SEQ ID NO:
• 52;
• CDR3 of the heavy chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 46, SEQ ID NO:
• 50.

A chimeric antigen receptor expressed on the surface of an immune cell comprising an antibody, wherein the chimeric antigen receptor comprises:

• the antibody, a transmembrane region and an intracellular signal region, which are sequentially linked;
• wherein the antibody specifically recognizing EGFRvIII expressed or EGFR overexpressed by tumor cells, wherein the antibody comprises a light chain variable region and a heavy chain variable region, and
• CDR1 of the light chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 41, SEQ ID NO:
• 47, SEQ ID NO:
• 55;
• CDR2 of the light chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 42, SEQ ID NO:
• 53;
• CDR3 of the light chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 43, SEQ ID NO:
• 48, SEQ ID NO:
• 54, SEQ ID NO:
• 56, SEQ ID NO:
• 57;
• CDR1 of the heavy chain variable region has the amino acid sequence of SEQ ID NO:
• 44;
• CDR2 of the heavy chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 45, SEQ ID NO:
• 49, SEQ ID NO:
• 51, SEQ ID NO:
• 52;
• CDR3 of the heavy chain variable region has an amino acid sequence selected from a group consisting of SEQ ID NO:
• 46, SEQ ID NO:
• 50.