Affinity support and method for trapping substance using the same
AssigneeBiomolecular Holdings LLC
InventorDaniel J. Capon
Life of this patent
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Abstract
Problems to be Solved The present invention provides an affinity support capable of trapping a substance by cooperative binding that is less likely to cause dissociation even when the substance is a molecule other than an antibody, and a trapping method using the same. Means to Solve the Problems A method of trapping a substance comprising the step of contacting an objective to be trapped with an affinity support comprising a support, a spacer bound to the support and an affinity substance bound to the spacer, so as to bind the objective to be trapped to the affinity substance, wherein each one of the objective to be trapped has a plural of affinity sites and the affinity substance binds to at least two of the affinity sites simultaneously.
Claims6
1-9. (canceled)
A method of trapping amyloid β comprising contacting a sample containing amyloid β (1-28) or amyloid β (1-42) with an affinity support which comprises (a) a solid support, (b) a plurality of spacers directly covalently bound to the solid support, (c) and a plurality of immunoglobulins, immunoglobulin F(ab′) fragments, or F(ab) fragments comprising the F(ab′) or F(ab) region of a 6E10 antibody molecule, each bound directly covalently to a spacer, wherein the length of the spacer is 1 to 20 nm and wherein the interval between affinity substance-binding positions is 1 to 50 angstrom (Å), wherein the amyloid β has a plurality of affinity sites and at least two of the affinity sites of the amyloid β are bound by the immunoglobulins, immunoglobulin F(ab′) fragments, or F(ab) fragments simultaneously such that the amyloid β (1-28) or amyloid β (1-42) is trapped by the immunoglobulin F(ab′) or F(ab) fragments via cooperative binding.
Dependencies are inferred from the claim text.