Peptides for inducing bacteriocin synthesis and methods to identify and/or select and/or optimize the same
AssigneesSyngulon SA, Université Catholique De Louvain
InventorsJohann Mignolet, Pascal Hols, Laura Ledesma García
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Abstract
Described herein is a peptide or peptidomimetic with a length of at least 6 residues comprising, consisting essentially of, or consisting of the sequence motif Xaa1-Trp-Xaa2-Xaa3-Xaa4-Xaa5 (SEQ ID NO:1), wherein: Xaa1 represents an aromatic residue (Phe, Tyr, Trp, His), Cys or Ser; Xaa2, Xaa3 and Xaa4 represent any residue; and Xaa5 represents Gly, lie or Val.
Claims22
A peptide or peptidomimetic able to induce bacteriocin production in a microbial organism with a length of at least 6 residues comprising the sequence motif Xaa1-Trp-Xaa2-Xaa3-Xaa4-Xaa5 (SEQ ID NO:1), wherein the sequence motif is located at the C-terminus of said peptide or peptidomimetic or located so that it is followed by 1, 2, 3, 4 or 5 additional C-terminal residues, and wherein:
- Xaa1 represents an aromatic residue (Phe, Tyr, Trp, His), Cys or Ser;
- Xaa2, Xaa3 and Xaa4 represent any residue;
- and
- Xaa5 represents Gly, Ile or Val.
A method for identifying, selecting and/or optimizing peptide ligands of peptide-responsive transcriptional regulators, comprising the following:
- a) generating a library of randomized genes, wherein said genes are operably linked to an inducible promoter, inducible by an inducer molecule;
- b) transforming the library into a microbial organism which comprises a nucleic acid encoding a selectable marker conferring resistance to a selection agent, operably linked to a promoter which is controlled by the peptide-responsive transcriptional regulator;
- and
- c) selecting or enriching positive clones by growing the microbial organism in the presence of the inducer molecule and the selection agent.
Dependencies are inferred from the claim text.