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US 2022/0017531 A1Published application
Fluorescent dyes
AssigneeCepheid
InventorsKevin P. Lund, Dmitri Sergueev, Alexander Gall
Life of this patent
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Abstract
Automated oligonucleotide synthesis-compatible fluorescent dye phosphoramidite compounds, solid supports, and labeled polynucleotides incorporating the compounds are provided. The compounds allow universal incorporation of the fluorescent label into any position of the polynucleotide.
Claims32
A compound represented by Formula I:
- or a stereoisomer, a salt, or a tautomer thereof,
- wherein:
- X is H, halogen, or C1-C05 alkyl;
- R1, R2, R3, and R4 are independently H or optionally substituted C1-C6 alkyl;
- R5, R6, R7, R8, and R9 are independently H, halogen, or optionally substituted C1-C6 alkyl;
- L1 is an optionally substituted C2-C10 alkylene or optionally substituted C2-C50 heteroalkylene;
- L2 and L3 are independently an optionally substituted C2-C10 alkylene or optionally substituted C2-C30 heteroalkylene;
- Q1 is a hydroxyl protecting group;
- Z is CH, N, NHC(O)N, or OC(O)N;
- Y is OH, OP(OCH2CH2CN)NR10R11, or a solid support;
- and
- R10 and R11 are independently optionally substituted C1-C6 alkyl.
9 more elements
Dependencies are inferred from the claim text.