Conjugates of bivalent evans blue dye derivatives and methods of use

A compound of Formula I or a pharmaceutically acceptable ester, amide, solvate, or salt thereof, or a salt of such an ester or amide or a solvate of such an ester amide or salt,

• wherein:
• R1, R2, R3, R4, R5, R6, R7, R8, R9, R10, and R11 are chosen independently from hydrogen, halogen, hydroxyl, cyano, C1-C6alkyl, C1-C6alkoxy, C1-C6haloalkyl, and C1-C6haloalkoxy;
• and
• L1 is -A1-B(A3)-A2-
• wherein A1 and A2 are chosen independently from a bond, —O—, —NH—, —NH(CO)—, —(CO)NH—, —NH(CH2)m(CO)— wherein m is an integer from 0 to 4, —(CO)(CH2)kNH— wherein k is an integer from 0 to 4, —NH(CS)NH—,
• A3 is —H, -halogen, —NH2, —SH, —COOH, or -L2-R12, wherein
• L2 is —(CH2)p— wherein p is an integer from 0 to 12, wherein each CH2 can be individually replaced with —O—, —S—, —NH—, —NH(CO)—, —(CO)NH—, —NH(CS)NH— provided that no two adjacent CH2 groups are replaced;
• R12 is —H, a chelating group, a crosslinker, or a conjugate;
• and
• B is
• or
• —(CH2)n- wherein n is an integer from 0 to 12, wherein each CH2 can be individually replaced with —O—, —NH(CO)—, or —(CO)NH— providing no two adjacent CH2 groups are replaced, and wherein —(CH2)n- is substituted with one substituent A3.