Systems and methods for on-chip analysis of nucleic acids and for multiplexed analysis of cells

Abstract

A microfluidic chip for on-chip detection of the presence or absence of a target nucleic acid region in an isolated nucleic acid sample is disclosed. The microfluidic chip includes a nucleic acid entanglement array, an isolated nucleic acid sample immobilized in the nucleic acid entanglement array, and at least one probe specific to a target nucleic acid region. Systems and methods of using the microfluidic chip are disclosed. An integrated microfluidic cell processing system is disclosed, which includes: a multiplexed microfluidic flow directing system having a plurality of reconfigurable microfluidic layers that form a plurality of reconfigurable microfluidic channels, where the multiplexed microfluidic flow directing system function to assist in directing flow of materials into, through, and out of the integrated cell processing system; and at least one microfluidic chip functionally integrated into at least one layer of the multiplexed microfluidic flow directing system, and operates under continuous flow conditions.

Dependencies are inferred from the claim text.