Aav virions with decreased immunoreactivity and uses therefor

A mutated adeno-associated virus (AAV) capsid protein comprising a substitution of one or more of the amino acids occurring at a position corresponding to a position of the full-length AAV-2 VP2 capsid, wherein the one or more amino acids are selected from the group consisting of amino acid:

• 124 substituted with a threonine;
• 126 substituted with an alanine;
• 127 substituted with an alanine or a leucine;
• 128 substituted with an alanine or an aspartic acid;
• 130 substituted with a threonine or an alanine;
• 131 substituted with a glutamine or an alanine;
• 132 substituted with a glutamic acid, an alanine, or an asparagine;
• 133 substituted with an alanine;
• 134 substituted with a glutamine, a phenylalanine, or an alanine;
• 188 substituted with an alanine;
• 190 substituted with an alanine;
• 191 substituted with a serine;
• 193 substituted with an alanine;
• 245 substituted with an alanine;
• 246 substituted with an alanine;
• 247 substituted with an alanine;
• 248 substituted with an alanine;
• 315 substituted with an alanine;
• 317 substituted with an alanine;
• 318 substituted with an alanine;
• 320 substituted with an alanine;
• 322 substituted with an alanine;
• 329 substituted with an arginine;
• 331 substituted with an alanine;
• 332 substituted with an alanine;
• 334 substituted with an alanine;
• 335 substituted with an alanine;
• 347 substituted with a cysteine;
• 350 substituted with a lysine;
• 354 substituted with an alanine;
• 355 substituted with a threonine or an alanine;
• 356 substituted with an arginine;
• 357 substituted with a glutamic acid, a glutamine, an alanine, or an asparagine;
• 358 substituted with a lysine;
• 359 substituted with an alanine;
• 360 substituted with a histidine, a lysine, or an alanine;
• 361 substituted with an alanine;
• 362 substituted with an alanine;
• 365 substituted with a phenylalanine or an alanine;
• 366 substituted with a serine or an alanine;
• 372 substituted with a glutamine, a lysine, a phenylalanine, or an asparagine;
• 375 substituted with a proline or an alanine;
• 377 substituted with an alanine;
• 390 substituted with an alanine;
• 391 substituted with an alanine;
• 392 substituted with a glutamic acid, a valine, an alanine, an asparagine, or an isoleucine;
• 393 substituted with a glutamine, a lysine, an alanine, or an aspartic acid;
• 394 substituted with a lysine or an alanine;
• 395 substituted with an alanine;
• 396 substituted with an alanine;
• 407 substituted with an alanine;
• 411 substituted with a lysine or an alanine;
• 413 substituted with a lysine or an alanine;
• 418 substituted with an alanine;
• 419 substituted with an alanine;
• 437 substituted with an alanine;
• 438 substituted with an alanine;
• 448 substituted with a lysine or an alanine;
• 450 substituted with a lysine or an alanine;
• 451 substituted with an alanine or lysine;
• 452 substituted with an alanine;
• 494 substituted with a proline;
• 568 substituted with a lysine or an alanine;
• 569 substituted with an alanine;
• 571 substituted with a lysine or an alanine;
• and
• 592 substituted with a lysine.