Hepatitis b virus vaccine and uses thereof
AssigneeJRHS Therapeutics Inc.
InventorsZhuang SU, Meijia YANG
Life of this patent
Estimating the term…
Abstract
A hepatitis B vims (HBV) vaccine particle is described, including a recombinant HBV surface antigen including L surface protein; optionally M surface protein; and optionally S surface protein; wherein the molar percentage of L surface protein to the sum of L, M, and S surface proteins is at least about 1 mole %, 8 mole %, 10 mole %, 20 mole %, 30 mole %, 40 mole %, or 50 mole %. Methods of making the same and methods of treating or preventing HBV infection in a subject using the same are also described.
Claims29
A hepatitis B virus (HBV) vaccine particle, comprising a recombinant HBV surface antigen comprising:
- L surface protein;
- optionally M surface protein;
- and
- optionally S surface protein;
- wherein the percentage of L surface protein in the L, M, and S surface proteins is at least about 1 mole %.
A recombinant nucleic acid sequence encoding L surface protein, wherein the recombinant nucleic acid sequence does not have an internal cis-element.
A method for preparing an HBV vaccine particle, comprising:
- a) providing recombinant expression vectors comprising a first, second, and third recombinant nucleic acid sequences encoding L, M, and S surface proteins respectively;
- and wherein the first, second, and third recombinant nucleic acid sequences do not have an internal cis-element;
- b) transforming cells with the recombinant expression vectors;
- and
- c) culturing and selecting for cells to co-express L, M, and S surface proteins.
Dependencies are inferred from the claim text.