Generation Of Uniform Hepatocytes From Human Embryonic Stem Cells By Inhibiting TGF-BETA and Methods Of Maintaining Hepatic Cultures
AssigneeWisconsin Alumni Research Foundation
InventorsJames A. Thomson, Srikumar Sengupta
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Abstract
This disclosure relates generally to new methods of maintaining the expression of hepatic genes in human hepatocytes and method for maintaining the functional hepatic enzyme activity of primary hepatocytes in culture. The disclosure also encompasses new methods of deriving a population of pure hepatocytes without selecting or sorting the cells from the cultured pluripotent cells.
Claims20
A method of expressing or inducing expression of one or more mature hepatic gene(s) in a human hepatocyte, the method comprising:
- culturing the human hepatocyte with at least one inhibitor of mitogen activating protein kinase (MAPK),
- wherein the cultured human hepatocyte expresses the one or more mature hepatic gene(s) at an expression level that is at least 70% the expression level of same gene in fresh primary hepatocytes.
A method of expressing or inducing expression of one or more mature hepatic gene(s) in a human hepatocyte, the method comprising:
- culturing the human hepatocyte with at least one inhibitor of c-MYC,
- wherein the cultured human hepatocyte expresses the one or more gene(s) at an expression level that is at least 70% the expression level of same gene in control hepatocytes.
Dependencies are inferred from the claim text.