Gene editing of reproductive hormones to reduce fertility in ictalurus punctatus
AssigneeAuburn University
InventorsRex A. DUNHAM, Zhenkui QIN, Guyu QIN
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Abstract
Disclosed are fish with impaired reproductive capacity and methods and compositions for producing the same. The reproductively impaired fish may include catfish, such as Ictalurus punctatus. The disclosed methods may be generally useful for mitigating environmental impact of escaped genetically engineered fish.
Claims14
A method for reducing fertility in a fish comprising modifying the DNA sequence of said fish's luteinizing hormone, gonadotropin-releasing hormone, or follicle-stimulating hormone gene.
A method for inducing modifications to a nucleic acid sequence in a living cell comprising electroporating at least two times to deliver at least one plasmid encoding at least one DNA nuclease.
A fish with a modified luteinizing hormone, gonadotropin-releasing hormone, or follicle-stimulating hormone gene such that functional luteinizing hormone, Gonadotropin-releasing hormone, or follicle-stimulating hormone gene product is no longer produced.
A DNA vector encoding at least one DNA nuclease that binds to the catfish luteinizing hormone, gonadotropin-releasing hormone, or follicle-stimulating hormone gene sequence.
Dependencies are inferred from the claim text.