Sterile fish
OwnerKarl J. Clark, Tad S. Sonstegard +1inventors
InventorsKarl J. Clark, Tad S. Sonstegard, Daniel F. Carlson
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Abstract
The present disclosure provides, at least, sterile fish and methods for producing sterile fish.
Claims30
A method of producing a sterile fish, the method comprising:
- fertilizing an egg with a sperm, wherein the egg is obtained from a female fish comprising a gene-edited homozygous alteration in the three prime untranslated region (3′-UTR) of a gene.
A cell comprising a heterozygous alteration or homozygous alteration in the three prime untranslated region (3′-UTR) of a gene, wherein the gene contributes to normal development and/or normal migration of primordial germ cells, wherein the gene is selected from nanos3/nanos/nanos1, dnd1/dnd, ddx4/vasa, dazl, tdrd7, grip2, CaOC1q, cxcr4/cxcr4b, ly75, nlk1, nanog, cpsf6/CFlm68, cxcl12/sdf1, kop, piwi/ziwi, oct4, bucky ball, cxcr7, granulito, hub, miR-430, mkif5Ba, oskar, and puf/puf-A.
A fish comprising a heterozygous alteration or homozygous alteration in the three prime untranslated region (3′-UTR) of a gene, wherein the gene contributes to normal development and/or normal migration of primordial germ cells, wherein the gene is selected from nanos3/nanos/nanos1, dnd1/dnd, ddx4/vasa, dazl, tdrd7, grip2, CaOC1q, cxcr4/cxcr4b, ly75, nlk1, nanog, cpsf6/CFlm68, cxcl12/sdf1, kop, piwi/ziwi, oct4, bucky ball, cxcr7, granulito, hub, miR-430, mkif5Ba, oskar, and puf/puf-A.
Dependencies are inferred from the claim text.