Lettuce variety 41-194 rz

The tissue culture as claimed in claim 6, wherein said cells or protoplasts of the tissue culture are derived from a tissue comprising a leaf, pollen, embryo, cotyledon, hypocotyl, meristematic cell, root, root tip, anther, flower, seed or stem.

A lettuce plant of grown from a seed of lettuce variety 41-194 RZ, a sample of seed of said variety having been deposited under NCIMB Accession No. 43775, further comprising a transgene.

A method for producing a seed of a 41-194 RZ-derived lettuce plant comprising (a) crossing a plant of lettuce variety 41-194 RZ, a sample of seed of which having been deposited under NCIMB Accession No. 43775, with a second lettuce plant, and (b) whereby seed of a 41-194 RZ-derived lettuce plant forms.

A method of introducing at least one new trait into a plant of lettuce variety 41-194 RZ comprising:

• (a) crossing a plant of lettuce variety 41-194 RZ, a sample of seed of which having been deposited under NCIMB Accession No. 43775, with a second lettuce plant that comprises at least one new trait to produce progeny seed, (b) harvesting and planting the progeny seed to produce at least one progeny plant of a subsequent generation, wherein the progeny plant comprises the at least one new trait, (c) crossing the progeny plant with a plant of lettuce variety 41-194 RZ to produce backcross progeny seed, (d) harvesting and planting the backcross progeny seed to produce a backcross progeny plant, and (e) repeating steps (c) and (d) for at least three additional generations to produce a lettuce plant of variety 41-194 RZ comprising at least one new trait and all of the physiological and morphological characteristics of a plant of lettuce variety 41-194 RZ, when grown in the same environmental conditions.