Compositions and methods of prognosis and classification for preeclampsia
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsBrice L. Gauilliere, Martin S. Angst, Nima Aghaeepour, David K. Stevenson, Xiaoyuan Han, Mohammad S. Ghaemi
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Abstract
Multiparametric analysis is performed at the single cell level of biological samples obtained from an individual during pregnancy to obtain a determination of changes in immune cell subsets, which changes include, without limitation, altered activation states of proteins involved in signaling pathways. Changes occur in signaling pathways of these immune cells that are predictive of propensity to develop preeclampsia in the pregnancy.
Claims20
A method for assessing propensity to develop preeclampsia for an individual during pregnancy, comprising:
- obtaining a cellular biological sample for analysis comprising immune cells from a patient during pregnancy at multiple points during the pregnancy,
- measuring single cell levels of signaling proteins in immune cell subset(s);
- determining whether changes in levels of activated signaling proteins associated with propensity to develop preeclampsia are present;
- and
- providing an assessment of the patient's prognosis for propensity to develop preeclampsia;
- and
- treating the individual in accordance with the prognosis.
(canceled)
A method for assessing propensity to develop preeclampsia for an individual during pregnancy, comprising:
- obtaining a cellular biological sample for analysis comprising immune cells from a patient during pregnancy from at least two timepoints during the pregnancy,
- measuring single cell levels of pSTAT5 in CD4+ T cells in the biological sample;
- determining whether the basal level of pSTAT5 in CD4+ T cells has decreased during the pregnancy, indicating a propensity to develop preeclampsia;
- and
- providing an assessment of the patient's prognosis for propensity to develop preeclampsia.
Dependencies are inferred from the claim text.