Disk elisa for quantitative analysis

A method for detecting and quantifying an analyte, the said method comprising:

• (a) immobilizing a first binding agent on a nitrocellulose membrane;
• (b) adding an analyte onto the nitrocellulose membrane placed inside a spin column or a filter assembly;
• (c) adding a second binding agent onto the nitrocellulose membrane column;
• (d) adding a wash-buffer to the spin column;
• (e) centrifuging the spin column for eliminating any excess and/or unbound first binding agent, analyte and/or second binding agent;
• (f) adding a substrate that interacts with the second binding agent to form a reporter species;
• and
• (g) quantifying the analyte by quantifying the reporter species with the help of a suitable detector.