Kits and methods for isolating protein from biological and environmental samples

A kit for removing one or more contaminants from a biological or environmental sample, the kit comprising

• (a) a first solution comprising 0.01-2 vol % non-ionic detergent, 1 to 200 mM Tris buffer, 1 to 300 mM of one or more inorganic salts, and 1-30 vol % polyol, wherein the one or more inorganic salts in the first solution is selected from sodium salts, potassium salts, calcium salts, magnesium salts, chloride salts, bicarbonate salts and sulfate salts;
• (b) a second solution comprising 1 to 6M inorganic salt, wherein the inorganic salt in the second solution is selected from sodium salts, potassium salts, calcium salts, magnesium salts, chloride salts, bicarbonate salts and sulfate salts;
• and
• (c) homogenizing beads that are not reactive with the biological or environmental sample.