Profiling of biological analytes with spatially barcoded oligonucleotide arrays
OwnerEswar Prasad Ramachandran Iyer, Tarjei Sigurd Mikkelsen +5inventors
InventorsEswar Prasad Ramachandran Iyer, Tarjei Sigurd Mikkelsen, Augusto Manuel Tentori, Rajiv Bharadwaj, Marlon Stoeckius, James Michael Chell, Cedric Uytingco
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Abstract
This disclosure relates to methods for spatial profiling of analytes present in a biological sample. Also provided are methods for using spatially barcoded substrates to detect a biological analyte in a cell culture, an organism, and organoid. Also provided are methods for using spatially barcoded substrates to detect the temporal profile of a biological analyte.
Claims31
1-84. (canceled)
A method of identifying the spatial location of an analyte in a biological sample, the method comprising:
- (a) contacting a plurality of analyte capture agents with a biological sample disposed on a substrate, wherein:
- an analyte capture agent of the plurality of the analyte capture agents comprises:
- an analyte binding moiety that binds specifically to the analyte,
- an analyte binding moiety barcode, and
- an analyte capture sequence, and
- the substrate comprises a plurality of capture probes, wherein a capture probe of the plurality of capture probes comprises a spatial barcode and a capture domain that hybridizes to the analyte capture sequence;
- and
- (b) determining the sequence of (i) the spatial barcode or a complement thereof, and (ii) the analyte binding moiety barcode, or a complement thereof, and using the determined sequences of (i) and (ii) to identify the spatial location of the analyte in the biological sample.
Dependencies are inferred from the claim text.