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US 2022/0010347 A1Published application
Modification of rna-related enzymes for enhanced production
AssigneeTranslate Bio, Inc.
InventorsBettina Strack-Logue, Frank DeRosa, Michael Heartlein
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Abstract
The present invention provides, among other things, methods and compositions for large-scale production of capped mRNA using SUMO-Guanylyl Transferase fusion protein.
Claims24
-6. (canceled)
A fusion protein, wherein the fusion protein comprises guanylyl transferase and a small ubiquitin-like molecule (SUMO) protein comprising 90% identity to SEQ ID NO:
- 8 and SEQ ID NO:
- 7.
-10. (canceled)
A vector encoding a fusion protein comprising guanylyl transferase protein and a small ubiquitin-like molecule (SUMO) protein.
A method to produce a guanylyl transferase by fermentation, comprising:
- a) culturing in a fermentation medium a microorganism that is transformed with at least one recombinant nucleic acid molecule comprising a nucleic acid sequence encoding a guanylyl transferase fusion protein that has an amino acid sequence that is at least 90% identical to SEQ ID NO:
- 8 and SEQ ID NO:
- 7;
- and b) collecting a product produced from the step of culturing.
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Dependencies are inferred from the claim text.