Dna polymerase mutants having enhanced template discrimination activity
AssigneeIntegrated Dna Technologies, Inc.
InventorsMark Aaron Behlke, Joseph A. Walder, Richard Owczarzy, Scott D. Rose, Joseph R. Dobosy, Susan M. Rupp
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Abstract
This invention relates to mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA polymerase of SEQ ID NO.:1, wherein the amino acid sequence of the mutant Taq DNA polymerase consists of substitutions at residue positions 783, 784, or a combination of 783 and 784 of the unmodified Taq DNA polymerase of SEQ ID NO.:1.
Claims32
A mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA polymerase of SEQ ID NO.:1, wherein the amino acid sequence of the mutant Taq DNA polymerase consists of substitutions at residue positions 783, 784, or a combination of 783 and 784 of the unmodified Taq DNA polymerase of SEQ ID NO.:1.
A mutant Taq DNA polymerase having an enhanced template discrimination activity compared with an unmodified Taq DNA polymerase, wherein the amino acid sequence of the mutant Taq DNA polymerase is selected from the group of the following selected substitutions:
- V783F, H784Q, or a combination of V783L and H784Q.
A mutant Taq DNA polymerase consisting of an amino acid sequence selected from a group consisting of SEQ ID NO:83, SEQ ID NO:85, SEQ ID NO:89, SEQ ID NO:147, SEQ ID NO:149, SEQ ID NO:155, SEQ ID NO:151, SEQ ID NO:153, SEQ ID NO:157, SEQ ID NO:159, SEQ ID NO:161, SEQ ID NO:
- 172, SEQ ID NO:
- 174, SEQ ID NO:
- 176, SEQ ID NO:
- 178, and SEQ ID NO:
- 180.
Dependencies are inferred from the claim text.