D-amino acid oxidative enzyme mutant and application thereof
AssigneeAbiochem Biotechnology Co., Ltd.
InventorsZhenhua TIAN, Zhanbing CHENG, Yanbing XU
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Abstract
Provided is a D-amino acid oxidative enzyme mutant. The sequence of the mutant comprises a sequence by mutating the 54th amino acid residue N, the 58th amino acid residue F, the 211th amino acid residue C, and the 213th amino acid residue M of the sequence shown in SEQ ID NO:1 or the sequence having at least 76% identity with SEQ ID NO:1. The D-amino acid oxidative enzyme mutant has a higher enzyme activity, enzyme activity stability and/or ammonium resistance than a mild D-amino acid oxidative enzyme mutant. Also provided is an application of the D-amino acid oxidative enzyme mutant in preparing 2-oxo-4-(hydroxymethylphosphinyl)butyric acid.
Claims18
A D-amino acid oxidase mutant having a sequence comprising mutations of amino acid residue N at position 54, amino acid residue F at position 58, amino acid residue C at position 211 and amino acid residue M at position 213 on SEQ ID NO:
- 1 or on a sequence having at least 76% identity with SEQ ID NO:
- 1;
- the D-amino acid oxidase mutant has higher enzyme activity, stability of enzyme activity and/or ammonium tolerance than wild-type D-amino acid oxidase.
Dependencies are inferred from the claim text.