Insecticidal proteins
AssigneeSyngenta Participations AG
InventorClarence Michael Reynolds
Life of this patent
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Abstract
Compositions and methods for controlling plant pests are disclosed. In particular, novel chimeric insecticidal proteins having toxicity to at least coleopteran insect pests are provided. Nucleic acid molecules encoding the novel insecticidal proteins are also provided. Methods of making the insecticidal proteins and methods of using the insecticidal proteins and nucleic acids encoding the insecticidal proteins of the invention, for example in transgenic plants to confer protection from insect damage, are also disclosed.
Claims30
A chimeric insecticidal protein that is toxic to an insect pest, comprising in an N-terminal to C-terminal direction (a) an N-terminal region comprising an amino acid sequence that corresponds to amino acid 1 to amino acid 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351 or 352 of (i) SEQ ID NO:1 or an amino acid sequence that has at least 80% identity to SEQ ID NO:1;
- or (ii) SEQ ID NO:2 or an amino acid sequence that has at least 80% identity to SEQ ID NO:2;
- or (iii) SEQ ID NO:3 or an amino acid sequence that has at least 80% identity to SEQ ID NO:3;
- or (iv) SEQ ID NO:4 or an amino acid sequence that has at least 80% identity to SEQ ID NO:4, fused to (b) a C-terminal region comprising an amino acid sequence that corresponds to amino acid 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352 or 353 to amino acid 488, 489 or 490 of (i) SEQ ID NO:1, or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:1;
- or (ii) SEQ ID NO:2 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:2;
- or (iii) SEQ ID NO:3 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:3, or comprises SEQ ID NO:3;
- or (c) an N-terminal region comprising an amino acid sequence that corresponds to amino acid 1 to about amino acid 363 of SEQ ID NO:17, fused to (i) a C-terminal region comprising an amino acid sequence that corresponds to about amino acid 347 to about amino acid 489 of SEQ ID NO:1 or an amino acid sequence having at least 80% identity to at least 99% identity to SEQ ID NO:1;
- or (ii) a C-terminal region comprising an amino acid sequence that corresponds to about amino acid 346 to about amino acid 488 of SEQ ID NO:2, or an amino acid sequence having at least 80% identity to at least 99% identity to SEQ ID NO:2.
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The method of 45, wherein the coleopteran insect pest is a Diabrotica species.
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A method of making a chimeric insecticidal protein comprising fusing in an N-terminal to C-terminal direction (a) an N-terminal region comprising an amino acid sequence that corresponds to amino acid 1 to amino acid 338, 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351 or 352 of (i) SEQ ID NO:1 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:1;
- or (ii) SEQ ID NO:2 or an amino acid sequence that has at least 80% identity of at least 99% identity to SEQ ID NO:2;
- or (iii) SEQ ID NO:3 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:3;
- or (iv) SEQ ID NO:4 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:4, fused to (b) a C-terminal region comprising an amino acid sequence that corresponds to amino acid 339, 340, 341, 342, 343, 344, 345, 346, 347, 348, 349, 350, 351, 352 or 353 to amino acid 488, 489 or 490 of (i) SEQ ID NO:1, or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:1;
- or (ii) SEQ ID NO:2 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:2;
- or (iii) SEQ ID NO:3 or an amino acid sequence that has at least 80% identity to at least 99% identity to SEQ ID NO:3;
- or c) an N-terminal region comprising an amino acid sequence that corresponds to amino acid 1 to about amino acid 363 of SEQ ID NO:17, fused to (i) a C-terminal region comprising an amino acid sequence that corresponds to about amino acid 347 to about amino acid 489 of SEQ ID NO:1 or an amino acid sequence having at least 80% identity to at least 99% identity to SEQ ID NO:1;
- or (ii) a C-terminal region comprising an amino acid sequence that corresponds to about amino acid 346 to about amino acid 488 of SEQ ID NO:2, or an amino acid sequence having at least 80% identity to at least 99% identity to SEQ ID NO:2.
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Dependencies are inferred from the claim text.