US 2022/0010322 A1 · GENE SILENCING VIA GENOME EDITING

Abstract

The present invention relates to methods and compositions for gene silencing by genome editing. In some embodiments, nucleases are provided selected from the group consisting of meganucleases (MNs), zinc-finger nucleases (ZFNs), transcription-activator like effector nucleases (TALENs), Cas9 nuclease, Cfp1 nuclease, dCas9-FokI, dCpf1-FokI, chimeric Cas9/Cpf1-cytidine deaminase, chimeric Cas9/Cpf1-adenine deaminase, chimeric FEN1-FokI, and Mega-TALs, a nickase Cas9 (nCas9), chimeric dCas9 non-FokI nuclease and dCpf1 non-FokI nuclease. Additionally, the present invention relates to methods and compositions for gene silencing by genome editing. Also provided are methods and compositions for rearranging a chromosome by genome editing.

Claims18

Dependent claims

1) A method of reducing expression a target gene comprised of:

a) Introducing into a cell a nuclease capable of site-directed DNA cleavage at a target genomic site;
b) Making two or more double strand cuts within a single target gene;
c) Selecting for a cell where the double strand cuts have been repaired with the intervening DNA inverted;
d) reducing expression of the target gene.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

17/297,527Filed 26 Nov 2019

Priority

CN PCT/CN2018/1191554 Dec 2018

Published

13 Jan 2022

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

C12N 15/8213
Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for…:…
C12N 15/82
Vectors or expression systems specially adapted for eukaryotic hosts: for plant cells , e.g. plant artificial chromosomes
C12N 15/8241
For plant cells , e.g. plant artificial chromosomes: phenotypically and genetically modified plants via recombinant DNA technology
C12N 2800/80
Nucleic acids vectors: vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Gene silencing via genome editing

Life of this patent

Gene silencing via genome editing

Life of this patent

Abstract

Claims18