Process for producing a purified rhabdovirus from cell culture
AssigneeBoehringer Ingelheim International GmbH
InventorsDethardt MUELLER, Alan Pardo GARCIA, Tanja Anneliese GREIN, Friedrich KAESS, Judy NG, Katharina PESTA, Sabrina SCHNEIDER, Jordan TURNBULL
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Abstract
The present invention relates to the field of upstream and downstream processing and provides a process for producing a purified rhabdovirus from a cell culture, preferably a purified oncolytic rhabdovirus and in particular a vesicular stomatitis virus, including pharmaceutical compositions comprising the rhabdovirus.
Claims47
A method of producing a rhabdovirus in a cell culture comprising the step of:
- (i) obtaining a rhabdovirus harvest from the cell culture by:
- a. adding directly to the cell culture a viral release agent, followed by clarifying the cell culture and recovery of the rhabdovirus harvest in the supernatant,
- OR
- b. subjecting the cell culture to a filtration step followed by rinsing of the filter with a viral release agent, and recovery of the rhabdovirus harvest in the supernatant.
A process for purifying a rhabdovirus from a cell culture infected with the rhabdovirus, comprising the step of:
- (i) obtaining a rhabdovirus harvest from the cell culture by:
- a. adding directly to the cell culture a viral release agent, followed by clarifying the cell culture, and recovery of the rhabdovirus harvest in the supernatant,
- OR
- b. subjecting the cell culture to a filtration step followed by rinsing of the filter with a viral release agent, and recovery of the rhabdovirus harvest in the supernatant.
Dependencies are inferred from the claim text.