Method for producing pluripotent stem cell having released differentiation resistance to mesendoderm
AssigneeKyoto University
InventorsKoji ETO, Akinori YUZURIHA
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Abstract
The present invention provides a method for producing a pluripotent stem cell released from resistance to differentiation into mesendodermal lineage, including subjecting a pluripotent stem cell to a treatment for activating an integrin β1-β-catenin signal transduction pathway (excluding contacting the pluripotent stem cell with laminin 421 or a fragment thereof and/or laminin 121 or a fragment thereof), and the like.
Claims21
A method for producing a pluripotent stem cell released from resistance to differentiation into mesendodermal lineage, comprising subjecting a pluripotent stem cell to a treatment for activating an integrin β1-β-catenin signal transduction pathway (excluding contacting the pluripotent stem cell with laminin 421 or a fragment thereof and/or laminin 121 or a fragment thereof).
A kit for evaluating resistance of a pluripotent stem cell to differentiation into a mesendodermal lineage and releasing the resistance of the pluripotent stem cell, comprising a reagent for measuring an expression level of integrin β1 in the pluripotent stem cell, and a culture container coated with laminin 511 or a fragment thereof corresponding to 0.005-0.25 μg/cm2 of laminin 511 E8 fragment, or laminin 511 or a fragment thereof corresponding to 0.03-0.07 μg/cm2 of laminin 511 E8 fragment.
(canceled)
A method for evaluating resistance of a pluripotent stem cell to differentiation into a mesendodermal lineage, comprising measuring an expression level of integrin β1 in the pluripotent stem cell.
Dependencies are inferred from the claim text.