Superfluids disruption of saccharomyces cerevisiae (yeast), cell wall disintegration into nanoparticles and fractionation into beta-glucans, chitin and mannans (mannoproteins)
AssigneeAphios Corporation
InventorTrevor Percival Castor
Life of this patent
Estimating the term…
Abstract
The present invention is directed to methods and apparatus for and products from disrupting, removing intracellular proteins, enzymes and nucleic acids, spray drying, lipid extraction, and making nanoparticles of Saccharomyces cerevisiae (yeast) cell wall followed by acid and/or enzymatic hydrolysis to produce Beta (β)-glucans, chitin and mannans (mannoproteins). The process and apparatus feature critical, supercritical, or near critical fluids for disruption of yeast and making yeast cell wall nanoparticles. The product materials retain full activity and are devoid of residual processing chemicals such as solvents, salts, or surfactants.
Claims20
A method for fractionating yeast cells, including the steps of:
- contacting yeast cells with SuperFhuids™ under pressure,
- rapidly releasing the saturated yeast slurry into a decompression chamber to release intracellular proteins, enzymes and nucleic acids;
- spray-drying the disrupted yeast cells by heating during decompression into a partially or fully evacuated decompression chamber;
- re-contacting the spray dried disrupted yeast cells with SuperFluids™ to extract lipids;
- rapid expansion of SuperFluids™ saturated spray-dried-disrupted yeast cells to produce yeast cell wall nanoparticles;
- and
- subjecting the nanoparticles enzymatic cleavage of yeast cell nanoparticles to produce Beta-glucans, chitins and mannans fractions.
A product produced by contacting yeast with a SuperFluids under pressure, including the steps of:
- rapidly releasing the saturated yeast slurry into a decompression chamber to release intracellular proteins, enzymes and nucleic acids;
- spray drying the disrupted yeast cells by heating during decompression and/or decompression into a partially or fully evacuated decompression chamber;
- re-contacting the spray dried disrupted yeast cells with SuperFluids to extract lipids;
- rapid expansion of SuperFluids saturated spray-dried-disrupted yeast cells to produce yeast cell wall nanoparticles;
- enzymatic cleavage of yeast cell nanoparticles to product Beta-glucans, chitins and mannans fractions, or;
- hydrolysis of yeast cell nanoparticles to product Beta-glucans, chitins and mannans fractions, or;
- enzymatic cleavage and hydrolysis of yeast cell nanoparticles to product Beta-glucans, chitins and mannans fractions.
A product derived from the fractionation of yeast, wherein the yeast is subjected to critical, supercritical, or near critical fluids for disruption of yeast cells.
Dependencies are inferred from the claim text.