Methods and devices for performing flow-through capture of low-concentration analytes
AssigneeCalifornia Institute of Technology
InventorsRustem F. ISMAGILOV, Travis S. SCHLAPPI, Stephanie E. MCCALLA, Nathan G. SCHOEPP
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Abstract
Methods and devices for detecting a low concentration analyte in a sample are provided herein. The methods include flowing a sample through a porous membrane coated with a capture matrix to capture the low concentration analyte. The methods also can include detecting the captured analyte, such as by performing in-situ amplification of the analyte.
Claims27
A method of detecting a low concentration analyte in a sample, the method comprising:
- a. flowing a sample comprising the low concentration analyte through a porous membrane coated with a capture matrix and thereby capturing analyte on the coated membrane;
- and
- b. detecting the captured analyte,
- wherein the analyte has a concentration within the sample of 500 entities/mL or less, and wherein the flowing is performed in 1 hour or less.
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A method of performing in-situ amplification on a sample, the method comprising:
- a. flowing the sample comprising a first concentration of an analyte through a porous membrane coated with a capture matrix in a container and thereby capturing analyte with the coated membrane to provide a captured sample comprising a second concentration of analyte which is 1000× or more than the first concentration;
- and
- b. amplifying the analyte within the container,
- wherein the flowing and amplifying are performed in 1 hour or less.
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A low concentration analyte capture device, the device comprising:
- a. a housing;
- b. a porous membrane coated with a capture matrix, said coated membrane operatively coupled to the housing and configured to capture and thereby concentrate analyte from a sample flowed therethrough by 1000× or more in 30 min or less.
Dependencies are inferred from the claim text.