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US 2021/0403893 A1Published application
Highly active s-cyanohydrin lyase and application thereof
AssigneeAbiochem Biotechnology Co., Ltd.
InventorsZhenhua TIAN, Zhanbing CHENG, Chuanmin SUN
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Abstract
The invention provides a highly active S-cyanohydrin lyase obtained by mutating an amino acid residue at position 103 of a wild-type cassava S-cyanohydrin lyase. The mutation can significantly increase an expression of a mutant enzyme in E. coli and does not require a decrease in temperature when induced. Further mutations at position 128 and other sites were performed to obtain mutants with increased catalytic activity.
Claims11
A mutated S-cyanohydrin lyase, which is mutated at one or more sites selected from the group consisting of:
- amino acid residue of position 103, amino acid residue of position 128, amino acid residue of position 2, amino acid residue of position 81, amino acid residue of position 149, amino acid residue of position 94, and amino acid residue of position 176, wherein the amino acid residues are numbered as shown in SEQ ID NO. 1.
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Dependencies are inferred from the claim text.