Live attenuated strains of foot and mouth disease modified by deoptimization and uses thereof
AssigneesThe United States of America as represented by the Secretary of Agriculture, Codagenix Inc.
InventorsTERESA B. DE LOS SANTOS, AIDA E. RIEDER, FAYNA C. DIAZ-SAN SEGUNDO, ANNA KLOC, JOHN R. COLEMAN, STEFFEN MUELLER, GISSELLE N. MEDINA
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Abstract
The present disclosure describes deoptimized foot and mouth viruses and their use for prophylactic and therapeutic treatment of mammalian subjects. The recombinant viruses provided herein include alterations in several genomic regions as well as Differentiating Infected from Vaccinated Animals (DIVA) markers.
Claims32
A deoptimized foot and mouth disease virus (FMDV) comprising a substituted genomic region, wherein the substituted genomic region comprises a nucleic acid at least 95% identical to SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, or SEQ ID NO:
- 7, and encodes the same polypeptide as SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, or SEQ ID NO:
- 7, respectively, or encodes the same polypeptide as SEQ ID NO:
- 1, SEQ ID NO:
- 2, SEQ ID NO:
- 3, SEQ ID NO:
- 4, SEQ ID NO:
- 5, SEQ ID NO:
- 6, or SEQ ID NO:
- 7, respectively, with up to 10 amino acid replacements, deletions or additions.
A deoptimized modified foot and mouth disease virus (FMDV) constructed by substituting the P2 domain, or the P3 domain with a codon deoptimized or codon-pair deoptimized region encoding the same protein sequence, or encoding a protein sequence with up to 10 amino acid replacements, deletions or additions, wherein the codon pair bias of the modified sequence is less than the codon pair bias of the parent FMDV, and is reduced by 0.05, 0.1, or 0.2.
Dependencies are inferred from the claim text.