Insect toxin delivery mediated by a densovirus coat protein
AssigneesUniversity of Florida Research Foundation, Inc., Iowa State University Research Foundation, Inc.
InventorsBryony C. Bonning, Mariah Kemmerer
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Abstract
A coat protein of Junonia coenia densovirus (JcDNV) is used to deliver attached peptide insect toxin across the gut epithelium of a fall armyworm, Spodoptera frugiperda. A fusion protein comprising VP4 attached to an insect toxin via a peptide linker is developed. A composition comprising a JcDNV coat protein attached to an insect toxin via a peptide linker can be used for insect pest control.
Claims19
A fusion protein comprising a carrier protein attached to a second peptide via a peptide linker, wherein the carrier protein is derived from a Junonia coenia densovirus (JcDNV) coat protein.
The fusion protein according to any one of the preceding claims, wherein the peptide linker is a protease-resistant linker.
The fusion protein according to any one of the preceding claims, wherein the second peptide comprises an insect toxin.
A recombinant DNA comprising a nucleic acid sequence encoding a fusion protein, wherein the fusion protein comprises a carrier protein attached to a second peptide via a peptide linker, and wherein the carrier protein is derived from a Junonia coenia densovirus (JcDNV) coat protein.
A method of controlling insect pests comprising feeding a fall armyworm, Spodoptera frugiperda, with a food source comprising a fusion protein comprising a JcDNV coat protein attached to an insect toxin via a peptide linker, thereby allowing the fusion protein to pass across the gut epithelium of the fall armyworm and act within the hemocoel of the fall armyworm after the fusion protein is ingested by the fall armyworm.
Dependencies are inferred from the claim text.