Rapid multiplexed serological test

A method of performing a multiplexed immunoassay for detecting multiple antigens, comprising:

• (a) obtaining a biological sample comprising immunoglobulins;
• (b) providing a substrate comprising a fluidic channel, wherein a plurality of different antigens are attached to the fluidic channel;
• (c) flowing the biological sample through the fluidic channel under conditions that permit the immunoglobulins in the biological sample to bind to an antigen attached to the fluidic channel at respectively different loci in the fluidic channel;
• (d) flowing a wash buffer through the fluidic channel to remove immunoglobulins that do not bind to an antigen or that bind to an antigen with weak affinity from the loci in the fluidic channel;
• (e) flowing a first probe specific for a first immunoglobulin type through the fluidic channel under conditions that permit the first probe to bind to first immunoglobulins that are bound to the antigens of the loci in the fluidic channel;
• (f) flowing a second probe specific for a second immunoglobulin type through the fluidic channel under conditions that permit the second probe to bind to second immunoglobulins that are bound to the antigens of the loci in the fluidic channel.

A method of performing a multiplexed immunoassay, comprising:

• (a) contacting a biological sample from a subject comprising a plurality of immunoglobulins with a plurality of optical ring resonators under conditions that permit immunoglobulins to bind to a plurality of antigens, wherein each optical ring resonator of the plurality of optical ring resonators comprises multiple copies of a single antigen, such that the plurality of optical ring resonators comprises a plurality of antigens;
• (b) contacting one or more probes specific to one or more immunoglobulin types with the immunoglobulins bound to the plurality of antigens on the optical ring resonators under conditions that permit the one or more probes to bind to the immunoglobulins;
• and
• (c) detecting changes in resonance wavelength for the plurality of optical ring resonators during the contacting step of step (a), step (b), or during both contacting steps (a) and (b).